2020-01-30 · The membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBST) for 2 h at room temperature, and then incubated overnight at 4 °C with primary antibodies (Abcam, Shanghai China) against B-cell lymphoma-2 (Bcl-2, ab32124), Bcl-2-associated X (Bax, ab32503), total poly (ADP-ribose) polymerase (PARP, ab74290), X-linked inhibitor of apoptosis (XIAP, ab21278

Then anti-VEGF antibody (1:200, Abcam), anti-NeuN (1:200, Millipore), anti-GFAP (1:2,000, Millipore) primary antibody were applied to the sections and incubated at 4°C overnight. .. Cy3 conjugated secondary antibody (anti-mouse, 1:800) or 488-conjugated secondary antibody (anti-rabbit, 1:800) was incubated with the brain cryosection for 2 h at room temperature.

was used to determine concentrations of lactate (Abcam, Abcam, ab13847), anti-mitochondrial ﬁssion protein 1 (Fis1; 1:1000, Abcam, ab71498), anti-mitofusin IRAK1 antibody (1:200, Abcam, ab238, MA, USA) and Caspase-3 antibody (1:150, Abcam, ab13847, MA, USA) were incubated with the sections at 4℃ overnight. The following day, after being washed with PBS buffer, the sections were incubated first with secondary antibodies for 1 hour at room temperature and then with horseradish peroxidase (HRP)-coupled streptavidin. アブカムは、抗体、elisaキットなど10万を超える試薬製品の提供、使いやすい製品検索、豊富な在庫と迅速な配送、最新技術情報の発信などを通じ、ライフサイエンス研究をサポートします。 The near-infrared dye IR-61 restores erectile function in a streptozotocin-induced diabetes model via mitochondrial protection Xiao-Feng Yue 1, Chong-Xing Shen 1, Jian-Wu Wang 1, Lin-Yong Dai 1, Qiang Fang 1, Lei Long 2, Yi Zhi 1, Xue-Ru Li 3, Ya-Wei Wang 2, Gu-Fang Shen 2, Zu-Juan Liu 2, Chun-Meng Shi 2, Wei-Bing Li 1 The detection was made using ECL detection kit (Abcam, ab65623, MA, USA) with 5 min incubation in a dark room. The detection reagent was drained and then, the membrane was exposed to a sheet of diagnostic film in a film cassette for 1 to 30 min . active Caspase 3 human, reacts with human, mouse, rat, fruit fly (drosophila melanogaster), indian muntjac, rhesus monkey, xenopus laevis and zebrafish (pmid 19823566)predicted to react with chinese hamster (100 identity with immunogen), dog (100 identity with immunogen) and pig (100 identity with immunogen) due to sequence homology 6044 | CHE E T AL. up-regulation of several osteogenic genes, including runt-related tran-scription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin.5 caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat.

Please check that this product meets your needs before purchasing. Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far. Knockout Tested Rabbit recombinant monoclonal Caspase-3 antibody [EPR18297]. Validated in WB, IP, IHC and tested in Mouse, Rat, Human. Cited in 43 publication(s). I am pleased to let you know that ab2302, ab13847, ab44976 and ab4051 have been tested on rat samples and in IHC-P and would be covered by our guarantee for use in this species and application.

Citations for ab13847 by Abcam 781 publications reference this Antibody We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far.

The detection reagent was drained and then, the membrane was exposed to a sheet of diagnostic film in a film cassette for 1 to 30 min . active Caspase 3 human, reacts with human, mouse, rat, fruit fly (drosophila melanogaster), indian muntjac, rhesus monkey, xenopus laevis and zebrafish (pmid 19823566)predicted to react with chinese hamster (100 identity with immunogen), dog (100 identity with immunogen) and pig (100 identity with immunogen) due to sequence homology 6044 | CHE E T AL. up-regulation of several osteogenic genes, including runt-related tran-scription factor 2 (Runx2), alkaline phosphatase (ALP) and osteocalcin.5 caspase3 (ab49822, Abcam), total caspase3 (ab13847, Abcam), E-cadherin (CST, Danvers, MA, USA) and β-actin (cat. no. 8457, Cell Signaling Technology) were added respectively for overnight incubation at 4°C.

Knockout Tested Rabbit recombinant monoclonal VCAM1 antibody [EPR5047]. Validated in WB, IP, ELISA, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 136 publication(s).

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Nov 14, 2018 Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team Citations for ab13847 by Abcam. 781 publications reference this Antibody. We have found 781 publications or academic papers that reference the product ab13847 (Anti-Caspase-3 antibody) supplied by Abcam so far.

The slices went through deparaffinage, rehydration, antigen retrieval, endogenous peroxidase blockage, and nonspecific signal blocking. Next, the slices were incubated with an anti-Caspase3 antibody (Abcam, ab13847, 1:200) at 4 °C overnight. 2020-09-25 · And then, slices were incubated with GRP78 (Abcam, ab21685, rabbit, 1: 2000) overnight at 4 °C. After incubation, slices were washed by PBS and incubated with FITC-labeled IgG (Abcam, ab74290, 1:2000) for 45 min at room temperature.
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Rabbit polyclonal DDX4 / MVH antibody.

Protein AG agarose was purchased from Pierce (Waltham, MA, USA). HEK293 (RRID:CVCL_0045) and 769-P (RRID:CVCL_1050) kidney 2020-12-01 · Briefly, the specimen was fixed in 10% formaldehyde and embedded in paraffin.
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anti-Caspase-3. (Abcam,ab13847), anti-Bcl-2. (Abcam,ab196495), anti-Bax ( Abcam,ab53154) ve TUNEL (In Situ Cell Death Detection Kit,. POD;. 11684817910.

Some customers have used this antibody successfully in IHC-P however our latest tests were unsuccessful and therefore we can no longer guarantee this application. Garcia C, Videla Richardson G, Dimopoulos N, Fernandez Espinosa D, Miriuka S, Sevlever G, et al.Human Pluripotent Stem Cells and Derived Neuroprogenitors Display Differential Degrees of Susceptibility to BH3 Mimetics ABT-263, WEHI-539 and ABT-199. Protein Names. Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1, CASP3_HUMAN. UniProt Code History.

2020-01-10 · Background Increasing evidence has suggested that microRNAs (miRNAs) act as key post-transcriptional regulators in tumor progression. Previous studies have confirmed that miR-17-5p functions as an oncogene in multiple cancers and contributes to tumor progression. However, the role and biological functions of miR-17-5p in the development of laryngeal squamous cell carcinoma (LSCC) still remain

Wild-type and Caspase 3 knockout samples (±staurosporine treatment) were subjected to SDS-PAGE.

I can confirm that ab13847 tested in IHC-P on adult rat brain in an Abreview. I can recommend to review this on the datasheet for further information. Lane 1: Wild-type HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 2: Wild-type HAP1 cell lysate Lane 3: Caspase-3 knockout HAP1 cell lysate + Staurosporine (1μM for 4h) Lane 4: Caspase-3 knockout HAP1 cell lysate Lanes 1 - 4: Merged signal (red and green). Green - ab184787 observed at 35 kDa. Red - loading control, ab8245, observed at 37 kDa. ab184787 was shown to recognise pro Caspase 3 ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature.